Screening of Symbiotic Bacteria for Biodiesel Production in Algae Growth
Keywords:
Stenotrophomonas maltophilia, Algae, Chlorella pyrenoidosa, Scenedesmus abundans, Symbiotic, rRNA
Abstract
A novel symbiotic bacterial strain was isolated from the algae culture and further analyzed for sequence identification to determine the nature of the species. In this study, 3- to 4-month-old algae were cultured under nonaxenic conditions. Samples were taken to determine the presence of cross contamination, such as bacteria and other species, in the culture system. Microscopic analysis confirmed the presence of C. pyrenoidosa and S. abundans in the culture system. Additionally, bacterial identification revealed that stable colonies survived in the culture system. Several purification of two isolated pure colonies were carried out, and growth curves were plotted for the isolated colonies (white and yellow colonies), which had maximum absorbance values of 0.467 and 0.154, respectively, at 660 nm. Moreover, the isolated bacterial colonies were cocultured with pure axenic cultures of C. pyrenoidosa and S. abundans. Studies have shown that yellow colonies support the growth of algae. Hence, isolated and purified yellow colonies were subjected to morphological, biochemical, 16S rRNA sequencing and FAME analyses via gas chromatography. This analysis confirmed that the isolated symbiotic bacterium was Stenotrophomonas maltophilia. The consensus sequence was deposited in the NCBI GenBank KX768757.
References
1.Addgene (2018) Protocols, Agarose Gel Electrophoresis. https://www.addgene.org/protocols/gel-electrophoresis/
2.Adegoke AA, Stenström TA, Okoh AI (2017) Stenotrophomonas maltophilia as an Emerging Ubiquitous Pathogen: Looking Beyond Contemporary Antibiotic Therapy. Front. Microbiol 8: 2276.
3.Banerjee A, Sharma R, Chisty Y and Banerjee UC (2002) Botryococcus braunii: A renewable source of hydrocarbons and other chemicals. Critical Rev. Biotechnol 22(3):245-279.
4.Barnwal BK, Sharma MP (2005) Prospects of biodiesel production from vegetable oils in India. Renew Sust Energ Rev 9:363-398.
5.Borowitzka Armin M, Moheimani Reza N (2013) Algae for Biofuels and Energy Developments in Applied Phycology 5. In: Borowitzka, M.A., Ed., Algae for Biofuels and Energy, Preface, Springer Dordrecht Heidelberg New York London.http://dx.doi.org/10.1007/978-94-007-5479-9
6.Borowitzka MA (1988) Vitamins and fine chemicals from microalgae. In: Borowitzka, M.A, Borowitzka, L.J, editors. Microalgal Biotechnology. Cambridge University Press, Cambridge 153-196 .
7.Brennan L, Owende P (2010) Biofuels from microalgae–a review of technologies for production, processing, and extractions of biofuels and coproducts. Renew Sust Energ Rev 14:557-577.
8.Brooke JS (2021) Advances in the Microbiology of Stenotrophomonas maltophilia. Clin. Microbiol. Rev 34: e0003019
9.Chellamboli C, Perumalsamy M (2014) Application of response surface methodology for optimization of growth and lipids in Scenedesmus abundans using batch culture system. RSC Advances 4 (42): 22129-2214.
10.Chellamboli C, Perumalsamy M, (2016) Synergistic Effect of Hormones and Biosolids on Scenedesmus abundans for Eliciting Total Biolipids. Water Environment Research 88 (11):2180-2190.
11.Chellamboli C, Perumalsamy M, (2017) Stenotrophomonas maltophilia Strain Identification using 16S sequence of rRNA based molecular technique. NCBI GenBank: KX768757. https://www.ncbi.nlm.nih.gov/nuccore/KX768757
12.Chisti Y (2007) Biodiesel from microalgae. Biotech. Adv 25:294-306.
13.Chisti Y (2012) Raceways-based production of algal crude oil. In: Posten, C., Walter, C. (Eds.), Microalgal Biotechnology: Potential and Production. deGruyter, Berlin 113-146.
14.Chisti Y (2013) Constraints to commercialization of algal fuels. J Biotechnol 167: 201-214.
15.Gajdács M, Urbán E (2019) Prevalence and Antibiotic Resistance of Stenotrophomonas maltophilia in Respiratory Tract Samples: A 10-Year Epidemiological Snapshot.Health Serv. Res. Manag. Epidemiol 6:2333392819870774.
16.Griffiths MJ, Harrison STL (2009) Lipid productivity as a key characteristic for choosing algal species for biodiesel production. J Appl Phycol 21:493-507.
17.Ho SH, Ye X, Hasunuma T, Chang JS, Kondo A (2014) Perspectives on engineering strategies for improving biofuel production from microalgae — a critical review. Biotechnol. Adv 32 (8):1448–1459.
18.Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M and Darzins A (2008) “Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54:621-639.
19.Huang XF (2016) Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides. Bioresour. Technol 206:141-149.
20.Illman AM, Scragg AH, Shales SW (2000) Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzyme Microb Technol 27(8): 631-635.
21.Jacquelyn S. Meisel, Elizabeth A. Grice (2017) Chapter 4 - The Human Microbiome. Editor(s): Geoffrey S. Ginsburg, Huntington F. Willard,vGenomic and Precision Medicine (Third Edition),Academic Press 63-77.
22.Jill E. Clarridge, III (2004) Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious Diseases. Clin Microbiol Rev. 17(4): 840–862.
23.Khan MS, Zaidi A, Wani PA, Ahemad M, Oves M (2009) Functional diversity among plant growth-promoting rhizobacteria. In: Khan MS, Zaidi A, Musarrat J (eds) Microbial strategies for crop improvement. Springer, Berlin, 105-132.
24.Klein-Marcuschamer D, Chisti Y, Benemann JR, Lewis D (2013) A matter of detail: assessing the true potential of microalgal biofuels. Biotechnol Bioeng 110:2317-2322.
25.Leung DYC, Wu X, Leung MKH (2010) A review on biodiesel production using catalyzed transesterification. Appl. Energy 87:1083-1095.
26.Maha A. Rakaz, Mohammed O. Hussien, Hanan M. Ibrahim (2021) Isolation, Extraction, Purification, and Molecular Characterization for Thermostable α-Amylase from Locally Isolated Bacillus Species in Sudan. Biochem Res Int 6670380.
27.Malcata FX (2011) Microalgae and biofuels: a promising partnership?. Trends Biotechnol 29:542-549.
28.Marousek J, Maroušková A, Gavurová B, Tuček D, Strunecký O (2023) Competitive algae biodiesel depends on advances in mass algae cultivation. Bioresource Technology 374:128802.
29.Pate RC (2013) Resource requirements for the large-scale production of algal biofuels. Biofuels 4:409-435.
30.Pettipher GL, Wang HH (2005) Microbiological Techniques. Editors: Paulworsfold Alan Townshend Colin Poole, In Encyclopedia of Analytical Science (Second Edition), Elsevier Academic Press, London, pp 16-24.
31.Ponnuswamy I, Soundararajan M, Syed PS (2013) Isolation and characterization of green microalgae for carbon sequestration, waste water treatment and bio-fuel production. Int J Bio-Sci Bio-Techno 5:17-26.
32.Ranjan R, Asha Rani, Ahmed Metwally, Halvor S. McGee, and David L. Perkins (2016) Analysis of the microbiome: Advantages of whole genome shotgun versus 16S amplicon sequencing”,Biochem Biophys Res Commun 469(4): 967–977.
33.Rodolfi L, Chini ZG, Bassi N, Padovani G, Biondi N (2009) Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng 102:100-112.
34.Rühl C (2008) Bp statistical review of world energy. London: BP.
35.Sanders ER (2012) Aseptic Laboratory Techniques: Plating Methods.J Vis Exp 63: 3064.
36.Tica S, Filipovic S, Zivanovic Pand Milovanovic B (2010) Test run of biodiesel in public transport system in Belgrade. Energ Policy 38:7014-7020.
37.Ulgiati S (2001) A comprehensive energy and economic assessment of biofuels: when green is not enough. Crc Crit Rev Plant Sci 20:71-106.
38.Vyas AP, Verma JL, Subrahmanyam N (2010) A review on FAME production processes. Fuel 89:1-9.
39.Walt Ream, Bruce Geller, Janine Trempy, Katharine Field (2013) Molecular Microbiology Laboratory, Second Edition. https://doi.org/10.1016/C2011-0-07599-3
40.Walt Ream, Bruce Geller, Janine Trempy, Katharine Field, (2013) Polymerase Chain Reaction and DNA Sequence Analysis of Bacterial Ribosomal RNA Genes. Editor(s): Walt Ream, Bruce Geller, Janine Trempy, Katharine Field, Molecular Microbiology Laboratory (Second Edition), Academic Press, pp 43-70,
41.Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173(2):697–703. http://doi.org/ 10.1128/jb.173.2.697-703.
42.World Energy Outlook, (2007). The International Energy Agency (IEA).
43.Zafar AM, Javed MA, Aly Hassan A, Mehmood K, and Sahle-Demessie E (2021) Recent updates on ions and nutrients uptake by halotolerant freshwater and marine microalgae in conditions of high salinity. J. Water Process Eng 44:102382
44.Zhang S, Zhang L, Xu G, Li F, Li X (2022) A review on biodiesel production from microalgae: Influencing parameters and recent advanced technologies. Front. Microbiol., Sec. Microbiotechnology 13:970028 doi: 10.3389/fmicb.2022.970028
2.Adegoke AA, Stenström TA, Okoh AI (2017) Stenotrophomonas maltophilia as an Emerging Ubiquitous Pathogen: Looking Beyond Contemporary Antibiotic Therapy. Front. Microbiol 8: 2276.
3.Banerjee A, Sharma R, Chisty Y and Banerjee UC (2002) Botryococcus braunii: A renewable source of hydrocarbons and other chemicals. Critical Rev. Biotechnol 22(3):245-279.
4.Barnwal BK, Sharma MP (2005) Prospects of biodiesel production from vegetable oils in India. Renew Sust Energ Rev 9:363-398.
5.Borowitzka Armin M, Moheimani Reza N (2013) Algae for Biofuels and Energy Developments in Applied Phycology 5. In: Borowitzka, M.A., Ed., Algae for Biofuels and Energy, Preface, Springer Dordrecht Heidelberg New York London.http://dx.doi.org/10.1007/978-94-007-5479-9
6.Borowitzka MA (1988) Vitamins and fine chemicals from microalgae. In: Borowitzka, M.A, Borowitzka, L.J, editors. Microalgal Biotechnology. Cambridge University Press, Cambridge 153-196 .
7.Brennan L, Owende P (2010) Biofuels from microalgae–a review of technologies for production, processing, and extractions of biofuels and coproducts. Renew Sust Energ Rev 14:557-577.
8.Brooke JS (2021) Advances in the Microbiology of Stenotrophomonas maltophilia. Clin. Microbiol. Rev 34: e0003019
9.Chellamboli C, Perumalsamy M (2014) Application of response surface methodology for optimization of growth and lipids in Scenedesmus abundans using batch culture system. RSC Advances 4 (42): 22129-2214.
10.Chellamboli C, Perumalsamy M, (2016) Synergistic Effect of Hormones and Biosolids on Scenedesmus abundans for Eliciting Total Biolipids. Water Environment Research 88 (11):2180-2190.
11.Chellamboli C, Perumalsamy M, (2017) Stenotrophomonas maltophilia Strain Identification using 16S sequence of rRNA based molecular technique. NCBI GenBank: KX768757. https://www.ncbi.nlm.nih.gov/nuccore/KX768757
12.Chisti Y (2007) Biodiesel from microalgae. Biotech. Adv 25:294-306.
13.Chisti Y (2012) Raceways-based production of algal crude oil. In: Posten, C., Walter, C. (Eds.), Microalgal Biotechnology: Potential and Production. deGruyter, Berlin 113-146.
14.Chisti Y (2013) Constraints to commercialization of algal fuels. J Biotechnol 167: 201-214.
15.Gajdács M, Urbán E (2019) Prevalence and Antibiotic Resistance of Stenotrophomonas maltophilia in Respiratory Tract Samples: A 10-Year Epidemiological Snapshot.Health Serv. Res. Manag. Epidemiol 6:2333392819870774.
16.Griffiths MJ, Harrison STL (2009) Lipid productivity as a key characteristic for choosing algal species for biodiesel production. J Appl Phycol 21:493-507.
17.Ho SH, Ye X, Hasunuma T, Chang JS, Kondo A (2014) Perspectives on engineering strategies for improving biofuel production from microalgae — a critical review. Biotechnol. Adv 32 (8):1448–1459.
18.Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M and Darzins A (2008) “Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54:621-639.
19.Huang XF (2016) Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides. Bioresour. Technol 206:141-149.
20.Illman AM, Scragg AH, Shales SW (2000) Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzyme Microb Technol 27(8): 631-635.
21.Jacquelyn S. Meisel, Elizabeth A. Grice (2017) Chapter 4 - The Human Microbiome. Editor(s): Geoffrey S. Ginsburg, Huntington F. Willard,vGenomic and Precision Medicine (Third Edition),Academic Press 63-77.
22.Jill E. Clarridge, III (2004) Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious Diseases. Clin Microbiol Rev. 17(4): 840–862.
23.Khan MS, Zaidi A, Wani PA, Ahemad M, Oves M (2009) Functional diversity among plant growth-promoting rhizobacteria. In: Khan MS, Zaidi A, Musarrat J (eds) Microbial strategies for crop improvement. Springer, Berlin, 105-132.
24.Klein-Marcuschamer D, Chisti Y, Benemann JR, Lewis D (2013) A matter of detail: assessing the true potential of microalgal biofuels. Biotechnol Bioeng 110:2317-2322.
25.Leung DYC, Wu X, Leung MKH (2010) A review on biodiesel production using catalyzed transesterification. Appl. Energy 87:1083-1095.
26.Maha A. Rakaz, Mohammed O. Hussien, Hanan M. Ibrahim (2021) Isolation, Extraction, Purification, and Molecular Characterization for Thermostable α-Amylase from Locally Isolated Bacillus Species in Sudan. Biochem Res Int 6670380.
27.Malcata FX (2011) Microalgae and biofuels: a promising partnership?. Trends Biotechnol 29:542-549.
28.Marousek J, Maroušková A, Gavurová B, Tuček D, Strunecký O (2023) Competitive algae biodiesel depends on advances in mass algae cultivation. Bioresource Technology 374:128802.
29.Pate RC (2013) Resource requirements for the large-scale production of algal biofuels. Biofuels 4:409-435.
30.Pettipher GL, Wang HH (2005) Microbiological Techniques. Editors: Paulworsfold Alan Townshend Colin Poole, In Encyclopedia of Analytical Science (Second Edition), Elsevier Academic Press, London, pp 16-24.
31.Ponnuswamy I, Soundararajan M, Syed PS (2013) Isolation and characterization of green microalgae for carbon sequestration, waste water treatment and bio-fuel production. Int J Bio-Sci Bio-Techno 5:17-26.
32.Ranjan R, Asha Rani, Ahmed Metwally, Halvor S. McGee, and David L. Perkins (2016) Analysis of the microbiome: Advantages of whole genome shotgun versus 16S amplicon sequencing”,Biochem Biophys Res Commun 469(4): 967–977.
33.Rodolfi L, Chini ZG, Bassi N, Padovani G, Biondi N (2009) Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng 102:100-112.
34.Rühl C (2008) Bp statistical review of world energy. London: BP.
35.Sanders ER (2012) Aseptic Laboratory Techniques: Plating Methods.J Vis Exp 63: 3064.
36.Tica S, Filipovic S, Zivanovic Pand Milovanovic B (2010) Test run of biodiesel in public transport system in Belgrade. Energ Policy 38:7014-7020.
37.Ulgiati S (2001) A comprehensive energy and economic assessment of biofuels: when green is not enough. Crc Crit Rev Plant Sci 20:71-106.
38.Vyas AP, Verma JL, Subrahmanyam N (2010) A review on FAME production processes. Fuel 89:1-9.
39.Walt Ream, Bruce Geller, Janine Trempy, Katharine Field (2013) Molecular Microbiology Laboratory, Second Edition. https://doi.org/10.1016/C2011-0-07599-3
40.Walt Ream, Bruce Geller, Janine Trempy, Katharine Field, (2013) Polymerase Chain Reaction and DNA Sequence Analysis of Bacterial Ribosomal RNA Genes. Editor(s): Walt Ream, Bruce Geller, Janine Trempy, Katharine Field, Molecular Microbiology Laboratory (Second Edition), Academic Press, pp 43-70,
41.Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173(2):697–703. http://doi.org/ 10.1128/jb.173.2.697-703.
42.World Energy Outlook, (2007). The International Energy Agency (IEA).
43.Zafar AM, Javed MA, Aly Hassan A, Mehmood K, and Sahle-Demessie E (2021) Recent updates on ions and nutrients uptake by halotolerant freshwater and marine microalgae in conditions of high salinity. J. Water Process Eng 44:102382
44.Zhang S, Zhang L, Xu G, Li F, Li X (2022) A review on biodiesel production from microalgae: Influencing parameters and recent advanced technologies. Front. Microbiol., Sec. Microbiotechnology 13:970028 doi: 10.3389/fmicb.2022.970028
Published
2024-07-30
How to Cite
[1]
CHELLAMBOLI, C., KANNA, R.D. and PERUMALSAMY, M. 2024. Screening of Symbiotic Bacteria for Biodiesel Production in Algae Growth. IIChE-CHEMCON. (Jul. 2024). DOI:https://doi.org/10.36375/prepare_u.iiche.a405.
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